Binding of polylysine and Toluidine Blue to deoxyribonucleoprotein
نویسندگان
چکیده
منابع مشابه
Studies on the accessibility of deoxyribonucleic acid in deoxyribonucleoprotein to cationic molecules.
The binding of deoxyribonucleoprotein to Toluidine Blue, to cetylpyridinium chloride and to polylysine of various molecular weights was studied to determine the percentage of free DNA phosphate groups in deoxyribonucleoprotein. Binding was measured by addition of these reagents to deoxyribonucleoprotein at a range of concentrations such that complete precipitation of the deoxyribonucleoprotein ...
متن کاملPhotosensitization of T2 coliphage with toluidine blue.
The photodynamic action of dyes upon biological systems has been reviewed by Blum (1941). Inactivation of bacteriophage by this process has been studied by Clifton (1931), Perdrau and Todd (1933), Burnet (1933), Morales (1941), and Pacheco and Morales (1941). More recently, Welsh and Adams (1954) and Yamamoto (1956, 1958) investigated the kinetics of photodynamic inactivation of coliphage. In t...
متن کاملSusceptibility of Candida albicans to photodynamic therapy using methylene blue and toluidine blue as photosensitizing dyes.
The increased resistance of Candida albicans to antibiotic therapy indicates the need for alternative treatments for oral candidiasis. Photodynamic therapy (PDT) has been researched as an alternative tool to inactivate pathogenic microorganisms. It uses a combination of a photosensitizer and a visible light source. This study evaluated the susceptibility of C. albicans to PDT and compared the e...
متن کاملToluidine blue binding by developing muscle tissue: assay and data on the mechanism involved.
The correlation of the basophilia of tissues with their nucleic acid content is one of the oldest and most widely applied chemical interpretations of histological staining reactions. The interaction of basic dyes with tissue constituents has been regarded for a long time, and without explicit qualification, as a salt formation. This interpretation was based essentially on the observation of sto...
متن کاملToluidine blue: A review of its chemistry and clinical utility
Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components, thereby staining tissues rich in DNA and RNA. It has found wide applications both as vital staining in living tissues and as a special stain owing to its metachromatic property. Toluidine blue has been used in vivo to identify dysplasia and carcinoma of the oral cavity. Use of toluidine blue in...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1971
ISSN: 0306-3283
DOI: 10.1042/bj1210025pb